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Making Giant Unilamellar Vesicles via

5to10μl of lipid solution in chloroform (Support Protocol 1) at concentration of 0.5to0.66mM(∼0.5 mg/ml) Up to 600 mM sucrose, or sterile deionized H 2O Platinum wires, 0.5- to 2.0-mm diameter Microdispensers with glass bores (Drummond), or Hamilton syringes Sealed chamber (e.g., vacuum desiccator) connected to dry nitrogen or vacuum source

Freeze Drying - an overview ScienceDirect Topics

6.5.2 Freeze-Drying. Freeze-drying is a process typically used to remove residual solvent from a material to produce a dry powder that can be easily loaded into a cell. The material is dissolved in solvent and frozen in a dry-ice bath. The solvent sublimates and is …

Crosstalk of low density lipoprotein and liposome as a ...

A 5-FUC has low water solubility, therefore, the dry film method was utilized to load 5-FUC into LDL. An aliquot of the chloroform solution of 5-FUC was dried down under a stream of nitrogen gas in a test tube. When the chloroform was completely removed, a thin dry film at the bottom of each test tube was formed.

Vacutherm Vacuum Heating and Drying Ovens

Catalog number: 51014551. Dry up to six times faster than when using conventional units. Thermo Scientific™ Vacutherm Vacuum Heating and Drying Ovens provide outstanding capabilities for fast and gentle drying. Excellent heat transfer and fast heating up minimize operation time. A double-paned safety-glass front window provides unique ...

Long-Term Storage of Lyophilized Liposomal Formulations ...

Dec 01, 2014 The moisture content of lyophilized samples shown in Table 1 was determined using a DL37 coulometric moisture analyzer (Mettler Toledo, Columbus, Ohio) using pyridine free vessel solutions (Photovolt Instruments, Inc., St. Louis Park, Minnesota). The samples were prepared in a glove box purged with dry nitrogen to avoid absorption of ambient air moisture.

Protocol - Soil Total Carbon and Nitrogen

Dec 20, 2019 Put about 50g of the sieved and well-mixed dry soil into a grinding container. Place one container in the center or three containers on the three outside pins in the shatterbox. Close the lid, set timer for 1 minute and press start. Transfer ~7 mL of pulverized soil from the grinding container into a labeled 7mL plastic vial.

Oven Validation Protocol (procedure) needed

Dec 30, 2021 If you are dry heat sterilizing, then you will probably have to verify sterility issues along with the functionality of the oven. It's important that your validation documentation follow any standard approach you have within your organization for validation, and to address the requirements established (hopefully) when the equipment was purchased.

Drying Oven Method vs. Halogen Moisture Analyzer

Download the Drying Oven vs. Halogen Moisture Analyzer Guide. The simple answer is yes, as long as the results obtained by the two methods are comparable. This means that it is necessary to show evidence that the results are equivalent within specific tolerances, which is not such a straightforward question to answer. This white paper guides ...

(PDF) Vacuum Drying: Basics and Application

Drying experiments were carried out at vacuum chamber pressures of 2.5, 5 and 10 kPa, and drying temperature ranging from 30 to 50 C. The effects of …

EIIP Vol. II: Chapter 16 Methods for Estimating Air ...

EIIP Volume II ii Disclaimer As the Environmental Protection Agency has indicated in Emission Inventory Improvement Program (EIIP) documents, the choice of methods to be used to estimate emissions depends on how the estimates

Vesicle Preparation via Extrusion - Boston University

Evaporate solvent off in hood under gas stream while turning flask to coat sides. Attach to vacuum pump with room temperature trap. Wrap in aluminum foil. Leave vacuum pump on overnight. Hydrate Lipid: Add 5 ml DI water. Place in oven at a temperature above the gel-liquid crystal transition temperature (Tc or Tm) of the lipid with the highest Tm.

Ovens - Fisher Sci

Gases and Gas Accessories; Paper Chromatography Products; Solid Phase Extraction Products; TLC Products; Chemicals. Chemicals. ... Liquid Nitrogen Storage Equipment; Refrigerators; Refrigerators and Freezer Accessories; ... Ovens 1 – 30 1132 . Industries. Government; Safety; Controlled Environments; Compounding Pharmacy Product Guide ...

Ovens - Fisher Sci

Gases and Gas Accessories; Paper Chromatography Products; Solid Phase Extraction Products; TLC Products; Chemicals. Chemicals. Acids; ... Liquid Nitrogen Storage Equipment; Refrigerators; Refrigerators and Freezer Accessories; ... Ovens; Ovens 1 – 30 1132 ...

Activation and expansion of human T ... - Nature Protocols

Jan 13, 2020 This protocol describes the facile assembly of APC-ms in ~4 h and rapid expansion or enrichment of relevant T-cell clones in 2 weeks, and is applicable for T-cell manufacturing and assay development.

Nitrogen Gas Purity Grades for Different Industry Uses NiGen

Jan 29, 2021 Gaseous nitrogen with purity levels ranging from 95-99% is useful in the oil and gas industry to reduce the risk of fires and explosions in associated processes. Chemical storage tank inerting and pipeline purging with gaseous nitrogen helps minimize the risk of sudden combustion of their contents. Pipeline maintenance services frequently use ...

Pharmaceutics Free Full-Text An Updated Risk ...

Jul 13, 2021 Liposomal formulation development is a challenging process. Certain factors have a critical influence on the characteristics of the liposomes, and even the relevant properties can vary based on the predefined interests of the research. In this paper, a Quality by Design-guided and Risk Assessment (RA)-based study was performed to determine the Critical Material …

Genetic dissection of clonal lineage relationships with ...

Jul 30, 2018 Liposome-encapsulated tamoxifen has previously ... wrapped in foil and placed in a 37 C oven for 24 h. ... was desiccated under a 5 kPa stream of nitrogen gas for 5 min, followed by vacuum ...

Nanotraps for the containment and clearance of SARS-CoV-2

Jun 02, 2021 The liposomal-based Nanotrap surfaces were functionalized with either recombinant ACE2 proteins or anti-SARS-CoV-2 neutralizing antibodies and phagocytosis-specific phosphatidylserines. ... solvent was slowly evaporated by heating the lipid solution at 55C to remove the solvent and further dried in a vacuum drying oven to produce a dried ...

On-chip microfluidic production of cell ... - Nature Protocols

Mar 29, 2018 This protocol describes an on-chip microfluidic approach for high-throughput production of cell-sized liposomes. Monodisperse and unilamellar liposomes are formed by hydrodynamic flow focusing and ...

Liposome production by microfluidics: potential and ...

May 19, 2016 This paper provides an analysis of microfluidic techniques for the production of nanoscale lipid-based vesicular systems. In particular we focus on the key issues associated with the microfluidic ...

Liposome Preparation - Avanti Polar Lipids

Method of Liposome Preparation. Properties of lipid formulations can vary depending on the composition (cationic, anionic, neutral lipid species), however, the same preparation method can be used for all lipid vesicles regardless of composition. ... (1mL), the solvent may be evaporated using a dry nitrogen or argon stream in a fume hood. For ...

Understanding the formation mechanism of lipid ... - PLOS

Nov 28, 2017 The concentration of lipid solution was 10 mg/mL DOPC containing 0.1 mol% of Rho-PE. DOPC and Rho-PE were dissolved in chloroform in a centrifuge tube and dry nitrogen gas was blown through to evaporate chloroform. To remove chloroform completely, the centrifuge tube was set in a desiccator and kept under vacuum by a rotary pump for overnight.

Protocol - Microbial Biomass C N - KBS LTER

Oct 04, 2021 Samples are incubated for 24 h in the chloroform vapor-saturated atmosphere. At the end of theincubation period the desiccator is vacuum air-flushed 8 times in order to remove the chloroform. Samples are removed from the desiccator, placed in quart wide mouth canning jars and sealed with lids fitted with rubber septa for gas sampling.

thermoscientific

Protocol table top ovens, the Advanced Protocol range boasts additional features providing even more flexibility and accuracy Maximum efficiency • Timer extends the automation options available to the user – Automate the oven to turn on or off at pre-set times – Weekly function allows to set on / off times for each day of the week ...

(PDF) Preparation of the Multifunctional Liposome ...

Recently, the multifunctional liposome-constituted microneedle arrays (LiposoMAs) have been proven to be an interesting vaccine adjuvant-delivery system (VADS) that are stable and can be ...

Preparation of Cationic Liposomes ... - Sigma-Aldrich

Remove the vial from the vacuum pump and immediately suspend in distilled water at twice the final lipid concentration. Bath sonicate the lipid dispersion to clarity (2-5 min). Add an equal volume of buffer (e.g., 308mM NaCl, 40mM Hepes, pH7.4), and sonicate further for 2 minutes. Solution may be passed through a 0.22m filter to sterilize.

Bonding PDMS Glass Protocol - Harvard University

Rinse in D.I. water and blow dry with compressed nitrogen. Dry-run to set the bonding parameters for plasma etcher. 3. Check that the inlet valves of O 2 and Ni are open (on the wall). 4. Turn on the vacuum pump. 5. Turn the main power on (red bottom) . 6. Switch to open vacuum toggle switch (labeled as VAC in front panel). And check the throttle

Making Giant Unilamellar Vesicles via

Sealed chamber (e.g., vacuum desiccator) connected to dry nitrogen or vacuum source Vacuum grease Teflon holder and spacers for ITO-coated plates (see Support Protocol 2) Oven with hole to introduce electrodes or heating bath (for lipid systems in which at least one component has a chain-melting transition temperature, T m, above room temperature)

Effects of moisture content on the storage stability of ...

Sep 01, 2009 The lipid mixture (containing α-tocopherol) was dried under a stream of nitrogen gas and placed under vacuum for more than 2 hours, subsequently resuspended in autoclaved, distilled HEPES buffer, and sonicated for 5 minutes immediately before use. The liposome preparation contained 12.59 M α-tocopherol, 603 M DC-Cholesterol, and 403 M DOPE.

Recommendations and Critical Factors in ... - AAFCO

the early literature (4) shows that a variety of ovens were used (vacuum, electrically heated, gas heated, water-jacketed, and steam heated); drying times varied from 2 – 20 hours and drying to constant weight; and temperatures used varied from 65 - 135C. The vacuum oven was used by most laboratories in the 1920s and 1930s. The vacuum oven

Freeze-Drying of Liposomes with Cryoprotectants and Its ...

Through the use of high throughput screening, a freeze drying cycle was optimised; ramp freezing from from 4C to -45C, followed by primary drying at …

Effects of moisture content on the storage stability of ...

under a stream of nitrogen gas and placed under vacuum for more than 2h, subsequently resus-pendedinautoclaved,distilledHEPESbuffer,and sonicated for 5min immediately before use. The liposome preparation contained 12.59mM a-tocopherol, 603mM DC-Cholesterol, and 403mM DOPE. Liposomes and DNA were mixed in equal volumes at a final HEPES buffer

Liu Brinker JACS supp info-revised CJB

vials was evaporated under a nitrogen flow in a fume hood and lipid films were formed. The vials were then stored in a vacuum oven at room temperature overnight to remove any residual chloroform. The samples were frozen at -20oC before use. To prepare liposomes, the vials were brought to room temperature and rehydrated by adding 1 mL of

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